CBE/ENGR 225 Faculty Seminar: Leonidas Bleris, Ph.D. Associate Professor, Department of Bioengineering Department of Electrical Engineering Center for Systems Biology University of Texas at Dallas
Leonidas Bleris, Ph.D.
Associate Professor, Department of Bioengineering
Department of Electrical Engineering
Center for Systems Biology
University of Texas at Dallas
Host: Otger Campas
Tuesday, January 26, 2016
ELINGS HALL 1601
*Light refreshments will be provided*
Mammalian Synthetic Biology: Networks and Circuit Design Principles
We present results at the interface of mammalian synthetic biology and genome editing towards gene therapy applications. We use a combination of computational, theoretical, and experimental approaches to study biological networks. Variable gene dosage and noise are major sources of fluctuations in gene product levels in both endogenous and synthetic circuits. We study a number of network topologies and exploit their properties to engineer robust systems. Additionally, we introduce a novel methodology to control the copies and residence time of a gene product delivered in host human cells but also selectively disrupt fragments of the delivery vehicle. Finally, we introduce a CRISPR-based mechanism to target heterozygous activating mutations in cancer cells and show reversal of drug resistance to a small-molecule inhibitor.